One Gene, Two Signals: Autocrine Signaling of Pre-cCF10 in the Maintenance of the Pheromone Inducible Conjugative Plasmid PCF10

One Gene, Two Signals: Autocrine Signaling of Pre-cCF10 in the Maintenance of the Pheromone Inducible Conjugative Plasmid PCF10

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pCF10 is a very stable plasmid. A mutation in the toxic peptide of the post-segregational killing (PSK) system suggests that the PSK system does not contribute to pCF10 stability. In contrast to another conjugative plasmid, pAD1, deletion of the PSK system from the pCF10 minimal replicon did not significantly affect pCF10 stability. Future studies will attempt to determine how CcfA 1-22 affects pCF10 maintenance and if CcfA1-22 may be contributing to the remarkable stability of pCF10. (Abstract shortened by UMI.)To confirm CcfAi.22 was correctly processed to mature cCFlO (CC1A13-19) an HPLC assay was used. The conditions used for the HPLC were similar to those used to purify cCFlO (Nakayama et ah, 1994). Only if mature cCFlO (CcfA.13-19) anbsp;...


Title:One Gene, Two Signals: Autocrine Signaling of Pre-cCF10 in the Maintenance of the Pheromone Inducible Conjugative Plasmid PCF10
Author: Valorie Mazeffa
Publisher:ProQuest - 2008
ISBN-13:

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